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After 2 freeze-thaw cycles, 5% 5-sulfosalicylic acid (SSA, 20 L) was added, and the samples were centrifuged at 8000 g for 10 minutes at 4C

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Beyond providing mechanical support, this unit regulates solute exchange that influences cartilage nutrition and metabolism

64 : 12871292 Nishinaka Y, Masutani H, Oka S, Matsuo Y, Yamaguchi Y, Nishio K, Ishii Y and Yodoi J (2004) Importin alpha1 (Rch1) mediates nuclear translocation of thioredoxin-binding protein-2/vitamin D(3)-up-regulated protein 1