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Description

Sliusar, M

One study using conditional TBK1 / in CD4 + T cells ( Tbk1 fl/fl :CD4-Cre mice) and stimulation with TCR and CD28, suggested that basal TBK1 is required for constitutive AKT turnover to prevent hyperactivation of AKT upon T cell activation (124)

The gating cells strategy was based on FSC-A/SSC-A profile, followed by selecting single cells population (FSC-A/FCH-H), and subsequently detecting fluorescently labeled cells (Alexa Fluor 488-A/PE-A)