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Serum was carefully separated and stored at 80 until analysis

Harmonic restraints of 50.0 kcal mol 1 2 were applied to the solutes, including the crystallographic ions, to allow solvent relaxation while preserving the maximum fidelity to the docking pose, and the Andersen temperature coupling scheme 81,82 was used to control and equalize the temperature

Since AOMs are indicated in combination with lifestyle interventions, all participants received dietary and physical activity counseling, aiming to achieve a 500-kcal deficit per day and 150 minutes of physical activity per week

3D multi-channel bi-functionalized silk electrospun conduits for peripheral nerve regeneration