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Consequently, the bacterias ability to maintain metabolic and homeostatic functions becomes compromised, aligning with previous findings indicating increased susceptibility to additional oxidative stress ( 5 Conclusion Our study highlights the profound metabolic perturbations induced by -lactam antibiotics in E.coli , elucidating distinct yet overlapping effects across different antibiotic classes

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The terminal hydroxyl group forms hydrogen bonds with the side chains of Gln72 and Gln164, and its replacement with an amine would significantly weaken the binding affinity

[6] [2] [3] Severe vitamin B 12 deficiency can damage nerve cells