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performed bulk RNA sequencing of TDLNs and demonstrated increased expression of gene pathways associated with antitumor immune response, inflammatory response, hypoxia, angiogenesis, extracellular matrix remodeling, and EMT when compared to CRT - controls ( These findings suggest that the TDLN is an important site for generating an anti-tumor T cell response, and while irradiation of the primary tumor may induce an antitumor immune response at the TDLN level, direct TDLN irradiation has a detrimental effect on anti-tumor immune activation