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Description

-actin Antibody (C4) HRP was purchased from Santa Cruz Biotechnology (sc-47778 HRP)

Animals were maintained normally on nematode growth medium (NGM) plates as described 43

10.3390/nu14204396 194 MahdiA.KovameesO.ChecaA.WheelockC

Non-hormonal alternatives like clonidine and gabapentin are also used but may lead to adverse effects such as sleep disturbances, nausea, constipation, and dizziness (Dehghan et al., 2022)