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After three 5-min washes in PBS, sections were incubated with the corresponding secondary antibody for 45 min

Design of in vivo Experiments The animals were separated into 4 groups according to the treatment schedule, as follows: saline (saline 0.9% + cecal ligation and puncture CLP/ n = 18 animals), Ceftriaxone (Cef 20 mg/kg + CLP/ n = 6 animals), aqueous extract of Ab (Ab 135 mg/kg + CLP, n = 18 animals) and sham (surgery control, n = 18 animals)

Filippin L, Magalhes PJ, Di Benedetto G, Colella M, Pozzan T (2003) Stable interactions between mitochondria and endoplasmic reticulum allow rapid accumulation of calcium in a subpopulation of mitochondria

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