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Description

The following reaction system was used: 1 ng pGEX4T-2 plasmid, 50 mM Tris-HCl (pH 7.8), 5 mM MgCl 2 , 10 mM 2-mercaptoethanol, 10 g/ml BSA, 1 ng forward primer FP1 and 1 ng reverse primer RP1, 20 M dNTP, 1 M dideoxynucleotides (ddNTP), 2 units DNA Polymerase I (Invitrogen), and ddH 2 O to the reaction volume of 100 l