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After 16 h of growth, cells were harvested via centrifugation and the resulting supernatant was harvested and sterilized using 0.22 m syringe filter sterilizing units (VWR, 76479-016) to remove residual cells

In this context, the subcutaneous LDopa infusion pump could offer an intermediate approach between the two conventional administration routes of LDopa, potentially mitigating some side effects

A549 cells were incubated with pneumococci for 3 hours at 37C and 5% CO2 to allow bacterial internalization, while the other cell lines were incubated for only 1 h with bacteria

However, this skin disorder is common in people with diabetes who inject insulin subcutaneously every day