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doi: 10.1016/j.cytogfr.2017.03.001 160 FranciscoVPinoJCampos-CabaleiroVRuiz-FernndezCMeraAGonzalez-GayMAet al

In the USA, approximately 6.1 million people aged 12 years or older were diagnosed with OUD in 2022, and 76,508 opioid-involved overdose deaths were reported over the 12-month period ending in February 2024 [2, 3]

Many state-of-art tools developed lack the efficiency to precisely measure the in vivo dynamics

After the density of cells reached 70%80%, pcDNA3.1(+)-GST plasmids were transfected using Lipo 2000 (Invitrogen) according to the manufacturers instructions