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Importantly, most data correlating oxidative stress markers with ALS progression has been obtained using mutSOD1 animal models, in which the mechanisms behind oxidative stress are easy to devise but which are a surrogate for only a small proportion of all ALS patients

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After washing with PBS for 3 times, cells were permeabilized with 0.1% Triton X-100 for 10 min and blocked with 5% normal goat serum with 0.01% Triton X-100 in PBS for 1 hr at room temperature

However, after 48 h the larvae were granular in appearance, some markedly, irregular in shape, and movement was reduced