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A 200 ug/kg or 2 ug/kg dose of BPC 157 was intraperitoneally injected 10 minutes post-injury followed by the return of animals to their cages and assignment to interval groups varying from days 7, 15, 20, 90, 180, and 360

Gonzlez-Montaa J-R, Escalera-Valente F, Alonso AJ, Lomillos JM, Robles R, Alonso ME (2020) Relationship between vitamin B 12 and cobalt metabolism in domestic ruminant: an update

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Then, lysates were heated with Laemmli sample buffer (final 1X composition: 20 mM Tris, pH 6.8, 2% sodium dodecyl sulfate (SDS), 6% glycerol, 1% -mercaptoethanol, 0.002% bromophenol blue) at 95 C for 5 min