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Pellets were thawed on ice for 1 h and lysed in 200 l 1% SDS in PBS supplemented with 1 protease inhibitors (cOmplete, EDTA-free Protease Inhibitor Cocktail, Roche, 11836153001) and shaken for 30 min at room temperature with 1:1,000 v/v benzonase nuclease (Sigma, E1014)

doi: 10.1016/j.tiv.2016.01.014 78 WangNZhouYFuCWangHHuangPWangBet al

A single 30 g dose of gluten caused a transient increase in cellobiose excretion in well-controlled patients with normalization within 1 week ( The barrier defect in CeD is hypothesized to contribute to diarrhea by a paracellular flux of water and solutes ( Two decades ago, increased zonulin protein concentration was shown in patients with active CeD by the group of Fasano ( ex vivo in biopsies of healthy volunteers and patients with quiescent CeD ( Comparison of the amino-terminal end of zonulin and the active fragment of Zot (98), corresponding to the amino acids 815 of this fragment, did not affect permeability, but pretreatment offered significant protection against the effect of subsequent treatment with purified Zot or zonulin (98)

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