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BT474 cells and PC3- hTERT cells were cultured in DMEM F-12 medium (Gibco, Invitrogen) with 10% FBS (Gibco, Invitrogen) and 1% PenicillinStreptomycin (Gibco)

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In hepatocytes, carboxyl terminus of HSC70-interacting protein (CHIP) interacts with NIK and TRAF3 and appears to act coordinately with TRAF3 to promote NIK degradation [44]