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Taken together, these findings suggest that serine-mediated one-carbon metabolism is also key for the metabolic alteration of GBM, especially in the tumor microenvironment of low glutamine, and demonstrate a previously unknown role for MTHFD2 in mediating glutamine-deprived stress by redox maintenance through one-carbon metabolism in GBM cells

Vitamin B6 in its less common form of pyridoxal 5-phosphate (PLP) may be less likely to cause neuropathy at high doses

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No significant decrease in the OCR was observed in 5 Seahorse-analyzed samples ( Supplemental Figure 6 )