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In each method solvents A and B comprising of water-acetonitrile-formic acid (90:10:0.1, v/v/v) and methanol-acetonitrile-formic acid (90:10:0.1, v/v/v), respectively were used

Typically, studies tend to be conducted In Vitro first, so within a lab or test tube environment, before moving on to In Vivo when it is deemed safe enough to do so