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DAP BII was purified using 35 to 70% ammonium sulphate precipitation and hydrophobic column chromatography using a HiPrep 16/10 Butyl column (GE Healthcare), desalted using a HiPrep 26/10 desalting column (GE Healthcare) and finally subjected to anion-exchange column chromatography using a Mono Q 5/50 GL column (GE Healthcare)

10.1016/j.phymed.2019.153154 41 IngoldI.BerndtC.SchmittS.DollS.PoschmannG.BudayK.et al (2018)

This leads to accumulation and impaired flow of these substances within the hepatocyte and canalicular lumen, causing distension and dilation

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