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AMPK activates autophagy either directly by phosphorylating mTORC1 and ULK1, or indirectly by regulating the expression of ATGs [13]

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Digestion was completed by heating the samples to 90 C for 20 min, followed by dilution into an equal volume of hydrogen peroxide (30% Aristar BDH) and incubation for ~30 min period, allowing for effervescence to stop

Sampling and analysis of carbon tetrachloride a Medium Sampling method Analytical method Detection limit Sample size Comments Reference Air aspiration velocity: 28 l/min MIRAN infrared 400 g/m 3 Lioy & Lioy, optical path: 20 m spectrometry 1983 Air direct injection GC with 2 ECD's 0.4 g/m 3 8 ml injected Lillian & in series (estimated) Singh, 1974 Air direct injection GC - ECD 0.2 g/m 3 2 ml injected BIT-SC, 1976 Air direct injection GC - ECD 0.06 g/m 3 5 ml injected Lasa et al., 1979 Air direct injection, methane GC - ECD 0.01 g/m 3 12 ml injected thorough purification of Makide & added carrier gas and apparatus Yokohata, 1983 required Air adsorption on Porapak-N GC - ECD 1 g/m 3 20 litres advantage of using Van Tassel et liquid desorption (methanol) methanol over CS 2 is the al., 1981 absence of a background signal in the ECD Air adsorption on activated GC - ECD 0.2 g up to 30 litres activated charcoal shown Morele et al., charcoal, liquid desorption can be sampled to be more efficient 1989 (ethanol) trichloroethylene trapping material than used as IS XADs, Tenax or Chromosorbs adsorption on activated GC - FID ca