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Then, 20 l of Protein A/G Plus-Agarose beads was added and incubated at 4 C overnight

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When 5GABA-A receptor PAM, MP-III-022 was used in rats in the VPA model, an increase in mRNA level of KCC2 was observed only in females treated with a dose of 1 mg/kg, while the mRNA level of Gabra5 and Nkcc1 remained unchanged across doses and animal sexes [58]