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CJC 1295 is an analogue of growth hormone-releasing hormone (GHRH)

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Nonspecific proteins were blocked with 5% skim milk in TBS-T (25 mM Tris (pH 7.6), 138 mM NaCl and 0.05% Tween-20) for 1 h and probed with primary antibodies (diluted 1:10001:5000)