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10.4081/1156 Summary Keywords APOE4 , Alzheimer's disease, skeletal muscle, mitochondria, proteomics, mice Citation Johnson CN, Lysaker CR, McCoin CS, Evans MR, Thyfault JP, Wilkins HM, Morris JK and Geiger PC (2024) Skeletal muscle proteome differs between young APOE3 and APOE4 targeted replacement mice in a sex-dependent manner

Common pitfalls with traditional methods are: Slow, labor-intensive and hard to scale Multiplexed panels (Luminex, MSD) require specialized instrumentation and complex workflows Connecting inflammatory signaling back to cell health data often means different assays, different plates, different days Questions & Solutions Which cytokines are my cells producing

We used IMOD and TrakEM software for 3D analysis of the regions of interest, and for model-making