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Animals were monitored daily for clinical signs, with regular measurements of body weight, food consumption and vital signs

The translation of preclinical to clinical testing for molecularly-based compounds is limited by multiple factors including the appropriate scheduling and toxicity of the agent, the interpatient and intratumoral variability of the expression of the molecular target and its role as a predictor of treatment response, and molecular crosstalk among redundant, parallel intracellular signaling pathways (Haffty and Glazer, 2003)

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