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After staining, sections undergo a series of alcohol dehydrations, clearing in xylene, and are then mounted with a neutral resin before being examined under a microscope (BDS400-FL, Ote, Chongqing) for histological evaluation

Moreover, this process has also been shown to affect both upstream- and downstream-related proteins (or genes), thereby exerting different effects on the intracellular microenvironment, which ultimately influences the outcome of ferroptosis (21)

Loss of these genes impaired the synthesis of heparan sulfate (HS) and chondroitin sulfate (CS), the two most abundant sulfated GAGs, reduced lipoprotein uptake, and sensitized diverse cancer cell lines to ferroptosis

Stamford (CT): Appleton & Lange