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[PMC free article: PMC4291174] [PubMed: 25470569] 442

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H.Escalante-AburtoA

Pyrimethamine selection was terminated on Day 5, and mice were administered 5-fluorocytosine (1 mg/mL, Sigma) via drinking water to eliminate the CRISPR plasmid carrying the dhfr/yfcu selection cassette (negative selection)