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Description

In this scenario, you would expect all of your protein to be in the elution, and none of it to be in the flow-through

doi: 10.1016/j.jad.2016.05.003

Briefly, 100-L of different concentration standards or diluted plasma samples (1:10) were added to the wells in duplicate, and the plate was incubated for 60 min at 37 C