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Our results are in agreement with previous studies performed in bovines, where oocytes subjected to heat stress before 12 h of maturation 49 , or even in the short term at the early stage of maturation, resulted in increased Annexin-V binding and oocytes that underwent early apoptosis compared with those in the control group 51

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The ERa/ER receptors trigger the phosphorylation of eNOS via activation of the Src/the extracellular signal-regulated kinases 1 and 2 (ERK1/2)/phosphatidylinositol 3-kinase (PI3K)/Akt signaling pathway, whereas the GPER-1 receptor does so through the Src/epidermal growth factor receptor (EGFR)/PI3K signaling pathway, leading to the rapid release of NO for the regulation of vascular tension