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The plates were incubated in the BioSpa at 37 C and 5% CO 2 for 4 days

All products are available in lyophilized (freeze-dried) powder form and are intended solely for in-vitro research, laboratory experimentation, and analytical applications

Since some db/db mice succumbed to this treatment probably due to RIF toxicity at 16 mg/kg, we reduced the dose to 8 mg/kg and treated the animals 3 times weekly through oral gavage from day-4 onwards until 8 weeks post-infection

There is not yet a proposed mechanism for how antibiotic treatment affects tight junction expression in specific regions of the brain in these studies, but it could be through changes in metabolite composition or cytokine involvement