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Since storage additives are devoid of amino acids, elevation in intracellular amino acids is consistent with proteolysis (e.g., via proteasomal degradation), a phenomenon that is mechanistically linked to (extravascular) hemolysis 45

Of note, GFAP is produced by activated astroglia that release ROS and pro-inflammatory mediators, creating a vicious cycle between oxidative stress and inflammation and hence playing a vital role in neuroinflammation with its subsequent neuronal apoptosis and hence neuronal damage (Colombo and Farina 2016)

Briefly, HK-2 cells were seeded in 96-well plates at a density of 5,000 cells per well and allowed to adhere overnight

J Am Chem Soc 116:67396744 Yang YH, Jing LH, Yu XL, Yan DD, Gao MY (2007) Coating aqueous quantum dots with silica via reverse microemulsion method: toward size-controllable and robust fluorescent nanoparticles