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Briefly, a RT-PCR-nested amplification was performed, and purified PCR products were quantified using the PicoGreen assay (Invitrogen, Carlsbad, CA, USA), and quality analysed using the BioAnalyzer DNA 1000 LabChip (Agilent, Santa Clara, CA, USA) prior to sequencing

Notably, the combination of MCT and valproic acid has been reported to be associated with liver failure

GCH1 expression can inhibit lipid peroxidation through two mechanisms: (1) GCH1 can generate BH 4 , which functions to diminish endogenous oxidative radicals

Duration: Most researchers run this combination for 12 to 16 weeks before taking a break to assess results and prevent desensitization