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doi: 10.1038/s41420-022-01156-5

Miura 2012)

Crypt culture media consists of Advanced DMEM (Gibco 12491015) that was supplemented with EGF 40 ng ml 1 (PeproTech 315-09), Noggin 200 ng ml 1 (PeproTech 250-38), R-spondin 500 ng ml 1 (Peprotech 315-32), B27 1X (Life Technologies 17504044), CHIR99021 3 M (LC laboratories C-6556), and Y-27632 dihydrochloride monohydrate 10 M (Sigma-Aldrich Y0503)

If you are still low, consider taking a good methylated B complex such as B Strong or if you arent absorbing B12, you can do a high dose sublingual form such as B12 Power Open Up Detoxification Pathways Heavy metal toxicity can lead to excessive sweating ( 7 )