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For statistical analysis, at least 15 nodule samples and 50 sectioned symbiosomes were examined for the wild-type and mutant lines (for sym33-3 , mutant nodules with bacterial release were analyzed)

reclassified 12 of them as Category 1 substances, the first regulatory step toward adding them to be compounded by pharmacies

Cutting-edge directions and future prospects of neuronal metabolism research Looking ahead, integrating multi-omics approaches such as 4-dimensional fast data-independent acquisition (4D-FastDIA) quantitative proteomics and targeted metabolomics [e.g., dissecting how Danggui-Shaoyao-San regulates the glycogen synthase kinase 3 beta (GSK3)/PGC1 signaling pathway to improve brain glucose uptake and mitochondrial complex function in AD models (Wu et al., 2024)] with computational modeling is unraveling systematic regulatory networks in neuronal energy metabolism, pinpointing critical nodes like pyruvate dehydrogenase complex phosphorylation and dynamic MCT2 transporter expression (Brown and Ransom, 2007)

The guide RNA sequence used for studies in Gpx4 -/- IECs was CGTGTGCATCGTCACCAACG, which targeted exon 1