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As youre doing your purification, it is normal for the blue color to fade or turn completely to white when his-tagged proteins are bound to the column

To assess the effect of BBR on allergic inflammation in allergic rhinitis in vivo and examine its mechanism of action, murine models of Dermatophagoides farinae (Derf)-sensitized allergic rhinitis were administered BBR (Derf + BBR) or untreated (Derf, only) at 10 g/mL [54]

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