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The redness window is shorter (often 30 to 60 minutes versus 2 hours on session one) because the user is no longer over-pressing

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The N2a cells were seeded in 96-well plates and treated with a range of concentrations of PILO including 2, 4, 8, 16, 32, 64, 128, and 256 mM for 48 h, and then analyzed the cell viability according to the manufacturer s instructions