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Mechanistically, the CERS6-derived ceramides induce mitochondrial fragmentation by interacting with mitochondrial fission factor, which contributes to the dysfunction of the organelle in mouse models of metabolic disease (98)

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After boiling for 5 min, the samples were loaded onto SDS-PAGE gels for western blot analysis, and detected using anti-LHCB5 antibody (Agrisera, AS09407, 1:2000 dilution) and anti-GFP antibody (Abmart, M20004, 1:5000 dilution)

Examples include the use of the BCG vaccine for treating urinary bladder cancer [9] and skin cancer like melanoma [10], as well as human papillomavirus and hepatitis B virus vaccines, as prophylactic vaccines against cervical cancer and hepatocellular carcinoma, respectively [8]