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In summary, the CPZ model has been shown to mimic many aspects of human demyelinating diseases such as MS, and to be a useful experimental approach to study these diseases and test potential therapies, especially after its standardization on C57BL/6 mice (Vega-Riquer et al., 2019

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Analyzing the floxed p2ry1 alleles revealed recombination rates between 6% and 33% in the brainstem (bs), cb, ctx, hippocampus (hc) and optic nerve (opt) with similar numbers for gabbr1 and tdTomato alleles (Fig

for example, after insertion of the needle and just before injection, the plunger rod can be withdrawn slightly to aspirate and verify the needle is not intravascular, inject the product slowly, and apply the least amount of pressure necessary