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Beyond Purification: Evolving Roles of Fusion Tags in Biotechnology PMC Frontiers The Evolution of Intein Based Affinity Methods as Reflected in 30 years of Patent History glutathione intein cleavage A) A proposed mechanism for thiol induced reactions at the FRET based N extein cleavage assay. (A) Expressed protein ligation David 2004 European Journal of Biochemistry Wiley Online Library
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