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To assess the effect of FMT on the microbiota and determine treatment efficacy, researchers collected faeces samples pre-treatment and at 4-, 8-, and 24-weeks post-treatment from the FMT patients

The proteins were then separated by 12% (w/v) SDS-PAGE and transferred onto a polyvinylidene fluoride (PVDF) membrane (Merck Millipore, USA) using wet transfer at 85 V for 1 h with a BioRad electroblotting apparatus