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J Gene Med 10(3):306315

Moreover, adherence to the MD not only positively impacts clinical parameters such as weight, waist circumference, hepatic fat accumulation, insulin resistance, alanine transaminase levels, gamma-glutamyl transferase, total cholesterol and triglycerides, but also beneficially influences inflammatory biomarkers, including adhesion molecules and cytokines [129]

However, it remains challenging to incorporate these tests into routine use due to their cost, complexity, time sensitivity, and the potential requirement for sophisticated equipment, substantial and well-handled sample volumes, as well as extensive technical training ( 4.3 Semen cryopreservation and oxidative stress Currently, sperm cryopreservation has become one of the essential techniques in assisted reproduction and fertility preservation