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Knockdown of AKT and MEK Cells (3.5 10 5 ) were transfected with 110 pmol of akt1 , akt2, mek1 , or mek2 DsiRNA (Integrated DNA Technologies) using INTERFERin in vitro siRNA transfection reagent (Polyplus) as instructed by the manufacturers protocol

When activated HSCs are treated with p38 MAPK kinase inhibitors, ROS, lipid hydroperoxide, GSH production and Nrf2 activity are unaffected

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Likewise, kinetic analyses of 2-oxoglutarate transport in rat liver mitochondria indicated the presence of a single MichaelisMenten component with kinetic parameters in the range of those reported previously for kidney mitochondria (Chen et al., 2000