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Limitations of the study The normal levels were established using Finish RedCross samples from assumingly healthy volunteers upon blood donation, and clinical pathology samples were collected from Auria Biobank material

The increased cycling time with AST might be attributed to its protective role against muscle damage or oxidative stress induced by exhaustive exercise challenge

After washing twice with phosphate buffer saline (PBS), the cells were fixed with 4% paraformaldehyde for 15 min at RT and then stained with 0.1% crystal violet (G1014, Servicrbio, China) for 30 min

405 nm, 488 nm, and 638 nm lasers were sequentially employed to excite DAPI, Mito-Grx1-roGFP2, and Alexa Fluor 647 fluorescence, respectively