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Although the development of the IVC environment is relatively complete, embryos produced in vitro have less developmental potential than embryos developing in vivo because they lack the protective maternal endocrine environment provided to embryos developing in vivo 19,32

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In particular, NMDAR activation has been extensively studied and found to be essential for retinal homeostasis but, at the same time, to cause neurodegeneration when overactivated ( Glycolysis Is the Energetic Support of Mller Glia Bioenergetic support is central to retinal homeostasis, as the retina is one of the most metabolically active tissues in the body ( Lactate Can Act as a Primary Energy Source The predominant glycolysis during normal conditions results in the aerobic conversion of glucose into lactate, which is thought to be shuttled to the RGCs

The band at 478 nm corresponds to the 11- cis -retinal chromophore bound to opsin via a protonated Schiff base linkage