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Samples were normalized to approximately 10 ng/L dsDNA with 80% v/v methanol, transferred into total recovery HPLC vials, and stored at 80 C for later LC-MS analysis

In the industrial context, although the use of organic solvents can increase product yield and permits efficient solid-liquid separation, there are also disadvantages, such as high cost and higher generation of polluting waste materials [12]

World Health Organization Retrieved

Tandem affinity purification results in more highly refined and stable complexes than single-tagged strategy alone, as this technique requires even more strict binding and elution conditions