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Ethylene generation kinetics are monitored via headspace gas chromatography (HS-GC)

Accordingly, we found that artificial elevation of glutathione levels may induce SA accumulation in tobacco leaves, either following a single infiltration of exogenous GSH or OTC or continuous exposure to OTC ( Figure 1B , Figures S1 and S2 ) Furthermore, although GSH and OTC treatments of SA-deficient NahG tobacco did not increase free SA levels, bound SA levels were unexpectedly (1.5 to 2-fold) higher in treated NahG plants

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Glutathione supports this process by protecting the mitochondria, which are the "powerhouses" of your cells where energy is produced