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The 4.3 kb adenoviral fragment contains early region 1 (E1) transcription units E1A and E1B, integrated into chromosome 19 of the host genome 61,62 , enabling viral gene expression and therapeutic protein overexpression under viral, mammalian, or synthetic promoters

In this sense, primary metabolites are produced for growth, development, and survival and consist of amino acids, sugars, vitamins, lipids, nucleotides, and carbohydrates, which have critical duties in different metabolic processes including respiration and nutrient consumption

Principal component analysis (PCA) was displayed as score plots of the first two components

Thus, methods such as enzymology, high-performance liquid chromatography, and radioisotope labeling are not suitable to investigate NADPH metabolism in live cells 21