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Using an established assay for CRISPR-based knock-in of sequences at the CLTA N-terminus (a highly expressed gene in HEK293T cells [Leonetti 2016] , neither our original split-wrmScarlet 11 sequence nor its mutant library enabled detectable complementation (Figure S10B, left panels)

YeHXueKZhangPChenRZhaiXLingLet al

Journal of Ethnopharmacology 2009 123(3) 452-8

97,98 Senescent T cells are characterized by a phenotypic shift with the downregulation of the costimulatory molecules CD27 and CD28 and the upregulation of the killer cell lectin-like receptor subfamily G and CD57