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Next, the cells were fixed using 4% paraformaldehyde (Wako) and permeabilized using PBS containing 0.5% Triton X-100

As a result, aged animals produced oocytes with spindle defects leading to misaligned chromosomes (that will induce impaired cell division)

doi: 10.3389/fnut.2022.899746 120 LynchC

125-127 Interestingly, some evidence suggests relationships between vitamin D deficiency and higher risks of heart disease and cognitive dysfunction may be due in part to abnormal sleep patterns and poor sleep quality