glutathione-s-transferase tag purification GST-tagged Protein Kit GST-tagged Protein Purification

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J Am Soc Nephrol (2017) 28:19916

Ltd., received travel funding from Nutrition Society of Australia, received consultancy funding from Nutrition Research Australia, and has received speakers honoraria from The Cancer Council Queensland and the Princess Alexandra Research Foundation

Then cDNA was synthesized using High-Capacity cDNA Reverse Transcription Kits (4374967, Applied Biosystems) with the accompanied random primers, as described previously ( The mRNA expression levels of given genes in the transfected cells with or without cell sorting enrichment were determined by qPCR using Taq388 mix (Du et al., 2022) on a QIAGEN Q-Rex machine (Qiagen, Germany)