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Importantly, commonly used in vitro BBB models, such as transwell systems and endothelial cell lines including RBE4, bEnd.3, and hCMEC/D3 monolayers, have important limitations, as they may overpredict permeability and do not fully recapitulate key in vivo BBB features, including physiological shear stress and multicellular neurovascular architecture (Katt and Shusta, 2020)

The whole process usually takes just a few minutes and happens in a clinical setting

Avoid direct exposure to light and frequent temperature changes to prevent premature peptide degradation

These relatively common critical care presentations are essential knowledge for anyone practicing in the ICU