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A doctor should be consulted if there are signs of exhaustion (e.g listlessness, loss of performance)

doi: 10.1038/nature14189 90 MunozMEidenschenkCOtaNWongKLohmannUKuhlAAet al

UP000030688, Taxonomy: 57266

Next, the cells on the bottom surface of the membrane were fixed with 4% paraformaldehyde for 10 min and then stained with 0.4% crystal violet solution for 10 min [37]