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2.5 Apoptosis analysis Apoptosis of HEK293T and HK-2 cells was assessed using an Annexin V pacific blue/propidium iodide (PI) staining

5-Amino-1MQ stands out among fat loss peptides due to its unique mechanism of action targeting nicotinamide N-methyltransferase (NNMT), an enzyme linked to fat storage and metabolic inefficiency

Homocysteine Remethylation and Methionine Regeneration Homocysteine can be recycled into methionine through two major enzymatic pathways: Methionine synthase (MS) utilizes 5-methyltetrahydrofolate (5-MTHF) from the folate cycle as a methyl donor, requiring vitamin B12 as a cofactor

Based on the results from the DPPH radical-scavenging assay, they determined that the phenolic compound 7 (3,4-dihydroxybenzaldehyde) had a strong antioxidant capacity equal to that of ascorbic acid